An Introduction to the Design and Behavior of Bolted Joints by John Bickford

By John Bickford

Supplying a broad-based assessment of the criteria affecting the layout, meeting and behavior of bolted joints and their parts in all industries, this paintings info numerous meeting techniques in addition to particular failure modes and techniques for his or her avoidance. This variation positive aspects fabric on: the touch stresses among bolt head or nut face and the joint; thread varieties, sequence and periods; the stiffness of raised face flange joints; and extra.

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The effect of the intronic sequences varies with their size, location, and orientation within the reporter gene constructs, and the cell type used for transfection. First evidence for a key role of the first intron in fibroblast-specific expression of Col1a1 was obtained from the MoV13 mouse mutant which was generated by infection with the Moloney virus [103, 104]. Accidental insertion of the virus into the first intron of the Col1A1 gene caused a complete block of Col1a1 transcription in fibroblasts.

Exon 1 of Col10a1 contains an 80–114 bp 5′ UTR sequences, exon 159–169 bp, while exon 3 with 2136–2900 bp codes for the majority of the triple helical part, the entire C-terminal NC1-1 domain and up to 939 (human) 3′ UTR sequences [387]. Nuclear run on experiments indicate that collagen X expression is regulated strictly at the transcriptional level [388]. As expected from the restricted temporal and spatial expression of type X collagen in the fetal cartilage growth plate [389, 390], strong positive and negative regulatory cisacting elements have been described in the promoter of collagen X genes, with major differences, however, between mammalian and chicken genes [387].

7 kb in fibroblast monolayer [19, 265], which are expressed in different ratios depending on the cell type. This ratio increases significantly under the influence of TGFβ [308] which also increases the half life of the mRNA that is normally 9–10 h. In fibroblasts cultured in three-dimensional collagen gels, however, only the smaller form of α1(I) mRNA is expressed [267, 268]. As in other mRNA species, the 3′ untranslated segment of collagen mRNA is responsible for the stability and the translation rate of the collagen mRNA [309].

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